Journal: The Japanese Dental Science Review

Article Title: The application of injectable platelet-rich fibrin in regenerative dentistry: A systematic scoping review of In vitro and In vivo studies ☆

doi: 10.1016/j.jdsr.2022.02.003

Figure Lengend Snippet: A summary of the included in-vitro studies regarding in the application of I-PRF in regenerative dentistry.

Article Snippet: Fujioka-Kobayashi (2020) , Periodontal Regeneration , To compare the growth factor release of C-PRF with that of I-PRF, and to investigate the regenerative properties of C-PRF and I-PRF on human gingival fibroblasts. , - hGFs , 300 g 5 min - (5702 Eppendorf, Hamburg, Germany) , Upper layer , G1) hGFs G2) hGFs + I-PRF G3) hGFs + C-PRF , Growth Factor Release of PDGF-AA, PDGF-BB, PDGF-AB, TGF-β, VEGF, EGF, and IGF-1 by ELISA (On 1 st , 3 rd , 7 th and 10 th Days): Over 10 days → The increase in the release of PDGF-AA, TGF-β1, and EGF from C-PRF compared with those from I-PRF were the most pronounced. hGFs Viability by Live/Dead Assay (At 24 h): Both C-PRF and I-PRF demonstrated excellent cell viability and biocompatibility. hGFs Migration Assay (At 24 h): G3 > G2 > G1 hGFs Proliferation Assay (On 1 st , 3 rd , and 5 th Day): Day 1 → G3 = G2 = G1 Day 3 → G3 > G2 > G1 Day 5 → G3 > G2 > G1 Gene Expression by RT-qPCR (On 3 rd Day): TGF-β → G3 = G2 > G1 PDGF → G3 > G1 Immunohistochemical Staining of COL1 (On 14 th Day): G3 > G2 > G1.

Techniques: Activity Assay, Isolation, Bacteria, Microbial Assay, Diffusion-based Assay, Expressing, Viability Assay, MTT Assay, Gene Expression, Control, Staining, CCK-8 Assay, Migration, Wound Healing Assay, Enzyme-linked Immunosorbent Assay, Alamar Blue Assay, Concentration Assay, ALP Assay, Immunohistochemical staining, Co-Culture Assay, Activation Assay, In Vitro, Transwell Assay, Comparison, Proliferation Assay, Cell Culture, Cell Adhesion Assay, Morphology Assay, Immunofluorescence, Biomarker Discovery, Infection